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Literature summary extracted from

  • Hu, J.; Xue, Y.; Lee, S.; Ha, Y.
    The crystal structure of GXGD membrane protease FlaK (2011), Nature, 475, 528-531.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.23.52 additional information wild type enzyme is not affected by 1,2-ethanedithiol dimethanesulfonate and dithiothreitol Methanococcus maripaludis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.23.52 expressed in Escherichia coli Rosetta 2 (DE3) cells Methanococcus maripaludis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.23.52 sitting drop vapor diffusion method, using 30% (w/v) PEG 300, 50 mM glycine (pH 9.5) and 100 mM NaCl Methanococcus maripaludis

Protein Variants

EC Number Protein Variants Comment Organism
3.4.23.52 E25C/I206C the mutant is proteolytically active Methanococcus maripaludis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.23.52 membrane
-
 Methanococcus maripaludis 16020
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.52 Methanococcus maripaludis A9A677
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.52 metal-affinity column chromatography and gel filtration Methanococcus maripaludis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.52 preflagellin FlaB2 + H2O
-
 Methanococcus maripaludis flagellin FlaB2 + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.23.52 FlaK
-
 Methanococcus maripaludis